Journal: Oncoimmunology

Article Title: Near-infrared duocarmycin photorelease from a Treg-targeted antibody-drug conjugate improves efficacy of PD-1 blockade in syngeneic murine tumor models

doi: 10.1080/2162402X.2024.2370544

Figure Lengend Snippet: In vivo therapeutic efficacy of CD25-targeted NIR-DPR in a MB49-luc tumor mouse model.

Article Snippet: HT2-A5E and EL4 were purchased from ATCC (Manassas, VA, USA), MB49-luc and Pan02-luc from GenTarget Inc (San Diego, CA, USA), MOC1 and MOC2 from Kerafast (Boston, MA, USA), and LL/2-luc from Imanis Life Sciences (Rochester, MN, USA).

Techniques: In Vivo

Journal: Oncoimmunology

Article Title: Near-infrared duocarmycin photorelease from a Treg-targeted antibody-drug conjugate improves efficacy of PD-1 blockade in syngeneic murine tumor models

doi: 10.1080/2162402X.2024.2370544

Figure Lengend Snippet: Early host immune responses to CD25-targeted NIR-DPR combined with PD-1 blockade in a MB49-luc tumor mouse model.

Article Snippet: HT2-A5E and EL4 were purchased from ATCC (Manassas, VA, USA), MB49-luc and Pan02-luc from GenTarget Inc (San Diego, CA, USA), MOC1 and MOC2 from Kerafast (Boston, MA, USA), and LL/2-luc from Imanis Life Sciences (Rochester, MN, USA).

Techniques:

Journal: Oncoimmunology

Article Title: Near-infrared duocarmycin photorelease from a Treg-targeted antibody-drug conjugate improves efficacy of PD-1 blockade in syngeneic murine tumor models

doi: 10.1080/2162402X.2024.2370544

Figure Lengend Snippet: Enhanced anti-cancer immunity was successfully established after CD25-targeted NIR-DPR combined with PD-1 blockade in a MB49-luc tumor mouse model.

Article Snippet: HT2-A5E and EL4 were purchased from ATCC (Manassas, VA, USA), MB49-luc and Pan02-luc from GenTarget Inc (San Diego, CA, USA), MOC1 and MOC2 from Kerafast (Boston, MA, USA), and LL/2-luc from Imanis Life Sciences (Rochester, MN, USA).

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Nuclear Factor-κB Overexpression is Correlated with Poor Outcomes after Multimodality Bladder-Preserving Therapy in Patients with Muscle-Invasive Bladder Cancer

doi: 10.3390/jcm8111954

Figure Lengend Snippet: NFκB signaling was up-regulated after irradiation (RT) and inhibited with the treatment of an NFκB inhibitor, SC75741, mitigating the proliferative capability of bladder cancer cells. ( A ) Images and quantification by densitometry and ImageJ of Western blots for NFκB transcription factor proteins (p65 and p50) (n = 3) (n = 3) and ( B ) colony formation assays of MB49 and T24 cancer cells after pre-treatment with or without SC75741 (5 μM) and/or irradiation (5 Gy) (n = 4). * p < 0.05.

Article Snippet: Human bladder urothelial carcinoma cell line, T24, and murine bladder tumor cell line, MB49, were purchased from the ATCC/Bioresource Collection and Research Center (BCRC).

Techniques: Irradiation, Western Blot

Journal: Journal of Clinical Medicine

Article Title: Nuclear Factor-κB Overexpression is Correlated with Poor Outcomes after Multimodality Bladder-Preserving Therapy in Patients with Muscle-Invasive Bladder Cancer

doi: 10.3390/jcm8111954

Figure Lengend Snippet: Blockade of NFκB signaling by RNA interference inhibited radiation (RT)-induced nuclear translocation of phosphor-p65 and the invasiveness capability of murine bladder cancer cells. ( A ) Immunofluorescence staining of phospho-p65 at 0, 1, and 3 h after RT (5 Gy) of MB49 vector-control (Ctrl) and MB49 NFκB knock-down (KD) cells. ( B ) MB49 control (Ctrl) and MB49 NFκB KD cells were seeded in Matrigel-coated inserts of Boyden chambers, treated without and with RT (5 Gy). After 24 h, the invading cells were viewed microscopically (high-power field, 200 ×, left panel). Invading cells were counted. * p < 0.05, Ctrl sham group vs. Ctrl RT group.

Article Snippet: Human bladder urothelial carcinoma cell line, T24, and murine bladder tumor cell line, MB49, were purchased from the ATCC/Bioresource Collection and Research Center (BCRC).

Techniques: Translocation Assay, Immunofluorescence, Staining, Plasmid Preparation, Control, Knockdown